The group followed a standard procedure in which they first grew the fungus in the lab for four days and then harvested small amounts of the culture for testing. They then took these pieces and grew them on glass beads for another two and a half days. At this point, they introduced one of three different bacterial species, Bacillus subtilis, Pseudomomas aeruginosa, or Escherichia coli. After another two days of incubation, they looked for any signs of competition. Although neither the E. coli nor the Pseudomonas was affected, the B. subtilis was inhibited giving the team the ability to move ahead.